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Related Experiment Video

Updated: May 4, 2026

Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery
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Human endothelial progenitor cells internalize high-density lipoprotein.

Kaemisa Srisen1, Clemens Röhrl2, Claudia Meisslitzer-Ruppitsch1

  • 1Center for Anatomy and Cell Biology, Department of Cell Biology and Ultrastructure Research, Medical University of Vienna, Vienna, Austria.

Plos One
|January 4, 2014
PubMed
Summary
This summary is machine-generated.

Human monocytic endothelial progenitor cells (EPCs) internalize high-density lipoprotein (HDL). Different pathways exist for HDL-cholesterol and HDL-cholesteryl oleate within EPCs, impacting lipid traffic.

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Area of Science:

  • Cell Biology
  • Lipid Metabolism
  • Vascular Biology

Background:

  • Endothelial progenitor cells (EPCs) are crucial for vascular repair and originate from hematopoietic stem cells or monocytes.
  • Intracellular lipid trafficking in EPCs, particularly involving high-density lipoprotein (HDL), is not fully understood.
  • Understanding HDL uptake and processing in monocytic EPCs is vital for elucidating their role in lipid homeostasis.

Purpose of the Study:

  • To investigate the uptake and intracellular trafficking of human high-density lipoprotein (HDL) and its associated cholesterol in human monocytic EPCs.
  • To differentiate the intracellular pathways of HDL-derived cholesterol versus HDL-derived cholesteryl oleate within EPCs.
  • To clarify the role of EPCs in HDL metabolism and lipid transport.

Main Methods:

  • Utilized fluorescence and electron microscopy to track labeled HDL particles and cholesterol surrogates (bodipy-cholesterol, bodipy-cholesteryl oleate).
  • Employed cytochemical labels and fluorochromes (horseradish peroxidase, Alexa Fluor® 568) for detailed visualization.
  • Applied photooxidation methods and electron tomographic reconstructions to analyze subcellular localization and trafficking dynamics.

Main Results:

  • Demonstrated HDL uptake and transport in EPCs within 0.5 to 4 hours, with HDL components found in endocytic vesicles, multivesicular bodies, and lysosomes.
  • Observed distinct intracellular trafficking routes: HDL-derived cholesterol localized to endosomes, Golgi, and trans-Golgi-network, while HDL-derived cholesteryl oleate accumulated in lysosomes, Golgi, lipid droplets, RER, and mitochondria.
  • Identified HDL-containing endosomes forming "strings of pearl" structures and accumulation near the trans-Golgi-network.

Conclusions:

  • Human monocytic EPCs internalize HDL through endocytic pathways.
  • EPCs exhibit differential intracellular processing of HDL-derived cholesterol and cholesteryl oleate, indicating distinct metabolic fates.
  • These findings provide new insights into the complex role of EPCs in lipoprotein metabolism and cellular lipid management.