[Recent progress on SELEX and its applications].

Area of Science:

• Biochemistry
• Molecular Biology
• Biotechnology

Background:

• Systematic Evolution of Ligand by Exponential Enrichment (SELEX) is an in vitro screening technology developed over the last two decades.
• SELEX integrates library technology with screening techniques to isolate specific nucleic acid molecules, known as aptamers.
• Aptamers function similarly to antibodies by binding to specific target substances.

Purpose of the Study:

• To provide a concise overview of the recent advancements in SELEX technology.
• To highlight the diverse applications of aptamers in the biomedical field.
• To summarize the progress and utility of SELEX in scientific research.

Main Methods:

• SELEX involves screening a library of nucleic acid molecules.
• The screening process relies on ligand-aptamer interactions to identify binding molecules.
• This method facilitates the selection of high-affinity aptamers for specific targets.

Main Results:

• SELEX technology has undergone rapid development and refinement.
• Aptamers generated through SELEX have demonstrated significant potential in various biomedical applications.
• The technology enables the isolation of nucleic acid ligands with high specificity and affinity.

Conclusions:

• SELEX is a robust and evolving technology for aptamer discovery.
• The applications of SELEX-derived aptamers are expanding across the biomedical landscape.
• Continued development of SELEX promises further innovation in molecular diagnostics and therapeutics.