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Expression of membrane-type matrix metalloproteinases in synovial tissue from patients with rheumatoid arthritis or

H Mitsui1, N Tsuchiya, S Okinaga

  • 1Department of Orthopedics, Mitsui Memorial Hospital , Tokyo , Japan.

Modern Rheumatology
|January 7, 2014
PubMed

Insights

Matrix metalloproteinases (MMPs) and membrane-type MMPs (MT-MMPs) are expressed in rheumatoid arthritis (RA) and osteoarthritis (OA) synovial tissue. These enzymes may contribute to joint matrix degradation in RA and OA.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Rheumatology

Background:

  • Rheumatoid arthritis (RA) and osteoarthritis (OA) are characterized by joint destruction.
  • Matrix metalloproteinases (MMPs) and membrane-type MMPs (MT-MMPs) are implicated in extracellular matrix degradation.

Purpose of the Study:

  • To investigate the expression of MT-MMP and MMP mRNAs in synovial tissue from patients with RA and OA.
  • To determine if MT-MMP expression differs between RA and OA.

Main Methods:

  • Northern blot analysis was used to examine mRNA expression.
  • Synovial tissue samples from five RA patients and five OA patients were analyzed.

Main Results:

  • Strong expression of MT1-MMP, MT3-MMP, MMP-1, and MMP-3 was observed in both RA and OA synovial tissue.
  • Weak expression of MT2-MMP and MMP-8 was detected, while MT4-MMP was not found.
  • No significant differences in MT-MMP mRNA expression were found between RA and OA patients.

Conclusions:

  • Synovial tissue in both RA and OA expresses various MT-MMPs and MMPs.
  • MT1-MMP, MT3-MMP, and potentially MT2-MMP may contribute to matrix degradation in RA and OA.
  • These findings suggest MT-MMPs as potential therapeutic targets for preventing joint destruction.

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