Related Experiment Video
Updated: May 4, 2026
A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells
Published on: July 3, 2013
A recombinant PPRE-driven luciferase bioassay for identification of potential PPAR agonists
Feng-Yuan Tsai1, Yi-Ting Cheng1, Tsui-Chun Tsou1
1Laboratory of Molecular Toxicology, Division of Environmental Health and Occupational Medicine, National Health Research Institutes, Zhunan, Miaoli, Taiwan.
Abstract:
A recombinant Huh7-PPRE-Luc cell line, carrying a peroxisome proliferator response element (PPRE)-driven luciferase gene, is an efficient tool for evaluation of potential peroxisome proliferator-activated receptor (PPAR) agonists. The cells exhibited a good dose-response induction in PPRE-driven luciferase activity by three subtypes of PPAR agonists as well as by a retinoid X receptor agonist, 9-cis-retinoic acid. Here, the bioassay is fitted into a 96-well plate format for high-throughput screening purpose.
