Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Stem Cell Niche01:26

Stem Cell Niche

5.0K
The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
5.0K
Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

4.5K
Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
4.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dosing Subcutaneous Immunoglobulin to Achieve Target Levels in Hematopoietic Cell Transplant Recipients.

Blood cell therapy·2026
Same author

Near infrared photo-bacterialflora modulation technology realized controlling periodontitis: modulation of disease-associated dysbiosis in oral microbiota using near infrared photo-antibacterial targeting therapy (NIR-PAT<sup>2</sup>).

Journal of translational medicine·2026
Same author

Influence of oral contraceptive/low-dose estrogen progestin discontinuation on the incidence of alveolar osteitis after third molar extraction: a retrospective study at a single center.

Nagoya journal of medical science·2026
Same author

Valemetostat is efficacious against recurrent adult T cell leukemia/lymphoma skin lesions and cutaneous graft-versus-host disease.

Transplantation and cellular therapy·2026
Same author

Real-World Treatment Patterns and Outcomes of Patients With Acute Myeloid Leukemia in Japan in the Pre-venetoclax Era.

Clinical lymphoma, myeloma & leukemia·2026
Same author

A case of long-term comprehensive rehabilitation culminated in weaning from a ventilator and regaining oral intake for a patient who underwent tracheostomy, mechanical ventilation, and alternative nutritional management because of type II respiratory failure after ascending aortic artificial vascular replacement.

Japanese journal of comprehensive rehabilitation science·2026

Related Experiment Video

Updated: May 4, 2026

A Mouse Distraction Osteogenesis Model
04:24

A Mouse Distraction Osteogenesis Model

Published on: November 14, 2018

6.0K

Stem cell-conditioned medium accelerates distraction osteogenesis through multiple regenerative mechanisms.

Yuji Ando1, Kohki Matsubara1, Jun Ishikawa1

  • 1Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.

Bone
|January 7, 2014
PubMed
Summary
This summary is machine-generated.

Human mesenchymal stem cell conditioned medium (MSC-CM) accelerates bone regeneration in a high-speed distraction osteogenesis (DO) model. MSC-CM contains key factors that promote cell recruitment and differentiation, speeding up skeletal tissue repair.

Keywords:
Bone marrow stromal cellsCell recruitmentDistraction osteogenesisEndothelial progenitor cellsSecretomeStem cell-conditioned medium

More Related Videos

An Efficient and Reproducible Protocol for Distraction Osteogenesis in a Rat Model Leading to a Functional Regenerated Femur
09:26

An Efficient and Reproducible Protocol for Distraction Osteogenesis in a Rat Model Leading to a Functional Regenerated Femur

Published on: October 23, 2017

6.7K
Mesenchymal Stromal Cell Culture and Delivery in Autologous Conditions: A Smart Approach for Orthopedic Applications
10:30

Mesenchymal Stromal Cell Culture and Delivery in Autologous Conditions: A Smart Approach for Orthopedic Applications

Published on: December 8, 2016

10.5K

Related Experiment Videos

Last Updated: May 4, 2026

A Mouse Distraction Osteogenesis Model
04:24

A Mouse Distraction Osteogenesis Model

Published on: November 14, 2018

6.0K
An Efficient and Reproducible Protocol for Distraction Osteogenesis in a Rat Model Leading to a Functional Regenerated Femur
09:26

An Efficient and Reproducible Protocol for Distraction Osteogenesis in a Rat Model Leading to a Functional Regenerated Femur

Published on: October 23, 2017

6.7K
Mesenchymal Stromal Cell Culture and Delivery in Autologous Conditions: A Smart Approach for Orthopedic Applications
10:30

Mesenchymal Stromal Cell Culture and Delivery in Autologous Conditions: A Smart Approach for Orthopedic Applications

Published on: December 8, 2016

10.5K

Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Stem Cell Biology

Background:

  • Distraction osteogenesis (DO) is effective for skeletal regeneration but requires lengthy treatment.
  • A high-speed DO (H-DO) model showed impaired bone callus formation.
  • Accelerating DO healing is crucial for clinical applications.

Purpose of the Study:

  • To investigate the efficacy of human mesenchymal stem cell conditioned medium (MSC-CM) in accelerating bone regeneration in an H-DO mouse model.
  • To identify the specific factors within MSC-CM responsible for promoting bone healing.

Main Methods:

  • Developed an H-DO mouse model with double the distraction speed of controls.
  • Administered serum-free MSC-CM locally into the distraction gap.
  • Performed secretomic analysis to identify key factors in MSC-CM.
  • Conducted functional assays to assess cell recruitment, differentiation, and proliferation.

Main Results:

  • MSC-CM significantly accelerated callus formation in the H-DO model.
  • Identified MCP-1/-3 and IL-3/-6 as critical factors in MSC-CM for recruiting bone marrow stromal cells (mBMSCs) and endothelial cells/progenitor cells (EC/EPCs).
  • Demonstrated that IL-3/-6 enhance osteogenic differentiation of mBMSCs, while MCP-1/-3 are essential for mBMSC recruitment.

Conclusions:

  • MSCs secrete a variety of trophic factors that enhance skeletal tissue regeneration.
  • MSC-CM effectively accelerates healing in a challenging H-DO model by promoting angiogenesis and osteogenesis.
  • Targeted use of MSC-derived factors holds promise for improving DO treatment efficiency.