Development of a quantitative Correlative Light Electron Microscopy technique to study GLUT4 trafficking
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Published on: December 7, 2017
Lorna Hodgson1, Jeremy Tavaré, Paul Verkade
1School of Biochemistry, Medical Sciences Building, University of Bristol, University Walk, Bristol, BS8 1TD, UK.
Abstract:
Correlative Light Electron Microscopy (CLEM) combines advantages of light microscopy and electron microscopy in one experiment to deliver information above and beyond the capability of either modality alone. There are many different CLEM techniques, each having its own special advantages but also its technical challenges. It is however the biological question that (should) drive(s) the development and application of a specific CLEM technique in order to provide the answer. Here we describe the development of a CLEM technique that is based on the Tokuyasu cryo immuno-gold labelling technique that has allowed us to quantitatively study GLUT4 trafficking.
