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Related Experiment Video

Updated: May 4, 2026

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice

Published on: February 3, 2023

Proteomic differences between white and brown adipocytes.

Wei-Qiang Chen¹, Lin Li, Gert Lubec

¹Department of Pediatrics, Medical University of Vienna, Waehringer Guertel 18-20, 1090, Vienna, Austria.

|January 7, 2014

Summary

This summary is machine-generated.

This study compared soluble protein levels in white and brown adipocytes, identifying five proteins increased in brown fat. These findings offer insights into adipocyte function and potential therapeutic targets.

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Isolating Brown Adipocytes from Murine Interscapular Brown Adipose Tissue for Gene and Protein Expression Analysis

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Last Updated: May 4, 2026

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice

Published on: February 3, 2023

Isolation and Differentiation of Primary White and Brown Preadipocytes from Newborn Mice

Isolation and Differentiation of Primary White and Brown Preadipocytes from Newborn Mice

Published on: January 25, 2021

Isolating Brown Adipocytes from Murine Interscapular Brown Adipose Tissue for Gene and Protein Expression Analysis

Isolating Brown Adipocytes from Murine Interscapular Brown Adipose Tissue for Gene and Protein Expression Analysis

Published on: March 12, 2021

Area of Science:

Biochemistry
Proteomics
Cell Biology

Background:

Protein level differences exist between white (WA) and brown (BA) adipocytes.
Limited proteomic studies exist, excluding mitochondrial proteins.

Purpose of the Study:

To compare soluble protein levels between WA and BA.
To identify differentially expressed proteins and their functional implications.

Main Methods:

Proteins extracted from WA and BA, soluble fraction analyzed by 2D gel electrophoresis.
Differential protein spots identified using nano-LC-ESI-MS/MS (CID and ETD modes).
Differential expression confirmed by immunoblotting; protein network analysis performed.

Main Results:

Five proteins (DJ-1, dihydrolipoyllysine-residue acetyltransferase, isocitrate dehydrogenase subunit alpha, electron transfer flavoprotein subunit alpha, immunoglobulin-binding protein 1) were increased in BA.
Differential expression validated by immunoblotting.
Generated protein network suggested a link to ubiquitination.

Conclusions:

Differential protein expression between WA and BA provides context for adipocyte biochemistry.
Identified proteins serve as potential targets for future functional and therapeutic studies.