A new T helper cell specificity within the pigeon cytochrome c determinant 95-104

A T cell hybridoma with unusual antigen specificity has been isolated from B10.BR mice immunized with pigeon cytochrome c. Using synthetic peptide analogues of the pigeon cytochrome c T cell determinant 81-104 we have determined that this hybridoma is specific for residues within the sequence 95-104. However, the mode by which this T cell hybridoma recognizes this determinant in the context of the I-Ek molecule differs from all previously described T cell clones in that it is not stimulated by the moth cytochrome c analogue 81-103.