Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Conservative Site-specific Recombination and Phase Variation

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

Recombinant DNA

Recombinant DNA

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

1H MR-based detection of human plasma metabolic alterations in clear cell renal cell carcinoma.

BJUI compass·2026

Same author

BCG vaccination mitigates tau pathology and restores cognitive function in PS19 mice.

bioRxiv : the preprint server for biology·2026

Same author

Metabolotheranostics of pancreatic cancer by targeting choline, glutamine, and glucose transporters with photoimmunotherapy.

Npj imaging·2026

Same author

Homeobox A10 in gastrointestinal malignancies: unraveling metastatic mechanisms and novel therapeutic opportunities.

Cancer metastasis reviews·2026

Same author

Fingerprinting the Metabolic and Lipidomic Landscapes of Pancreatic Ductal Adenocarcinoma with MALDI Imaging.

Clinical cancer research : an official journal of the American Association for Cancer Research·2026

Same author

A longitudinal analysis of radiosensitizer development in cancer therapy.

Cancer letters·2026

Search research articles

Related Experiment Video

Updated: May 4, 2026

Recombineering Homologous Recombination Constructs in Drosophila

Recombineering Homologous Recombination Constructs in Drosophila

Published on: July 13, 2013

Direct facile screening of recombinant DNA vector constructs.

Paul T Winnard¹, Rushi Challa¹, Zaver M Bhujwalla²

¹Division of Cancer Imaging Research, Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.

Analytical Biochemistry

|January 8, 2014

Summary

This study introduces pRedScript, a novel method for easily identifying bacterial transformants. Red colonies signal successful transformation, simplifying recombinant DNA selection and promoter screening.

Keywords:

Recombinant DNA Red color Screening mCherry

More Related Videos

Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila

In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila

Published on: August 20, 2019

Related Experiment Videos

Last Updated: May 4, 2026

Recombineering Homologous Recombination Constructs in Drosophila

Recombineering Homologous Recombination Constructs in Drosophila

Published on: July 13, 2013

Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila

In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila

Published on: August 20, 2019

Area of Science:

Molecular Biology
Biotechnology
Genetics

Background:

Bacterial transformation screening relies on visual cues like colorimetry or fluorescence.
Efficient selection of recombinant DNA is crucial for molecular biology applications.
Current methods can be limited in sensitivity or ease of use.

Purpose of the Study:

To develop a facile and efficient method for screening bacterial transformants.
To introduce a bright red fluorescent protein reporter for visual identification of recombinant colonies.
To enable sensitive screening of bacterial promoters for optimizing fermentation.

Main Methods:

Constitutive expression of a bright red fluorescent protein (RFP) in bacterial transformants using the pRedScript system.
Visual inspection of bacterial colonies on agar plates under ambient white light.
Utilizing RFP fluorescence for sensitive detection of bacterial promoter activity.

Main Results:

pRedScript enables straightforward visualization of red bacterial colonies against a white background.
Red fluorescence serves as a clear indicator of successful transformation.
The system demonstrates sensitivity for screening bacterial promoters in fermentation optimization.

Conclusions:

pRedScript provides a simple, visual, and efficient method for bacterial transformant screening.
The bright red fluorescence reporter facilitates recombinant DNA selection and promoter analysis.
This system enhances molecular biology workflows and fermentation development.