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Related Experiment Videos

[Possibilities to elevate the factor VIII yield].

K H Uteg, K Tausendfreund

    Folia Haematologica (Leipzig, Germany : 1928)
    |January 1, 1987
    PubMed
    Summary

    Intensifying the collection of coagulation factor VIII (FVIII) involves optimizing donor selection, plasmapheresis, thawing techniques, and anticoagulant use. Donor conditioning with DDAVP shows significant potential for increasing FVIII yield.

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    [Dependence of factor VIII activity on blood group].

    Die Pharmazie·1985

    Area of Science:

    • Biochemistry and Biotechnology
    • Hematology
    • Process Engineering

    Context:

    • Haemophilia A treatment relies on coagulation factor VIII (FVIII) substitution therapy.
    • Manufacturing and demand for FVIII preparations necessitate efficient collection methods.
    • Understanding FVIII structure and function is crucial for optimizing its production.

    Purpose:

    • To present methods for increasing factor VIII collection through intensive measures.
    • To evaluate the impact of various techniques on FVIII yield.
    • To explore potential combinations of measures for enhanced FVIII collection.

    Summary:

    • Evaluated measures include donor testing/selection (+25%), plasmapheresis, improved thawing techniques (+20-30%), citrate-free anticoagulants (+30%), and donor conditioning with DDAVP (+200-400%).
    • These strategies focus on optimizing both the biological source material and processing variables.
    • Combinations of these measures can significantly intensify factor VIII collection.

    Impact:

    • Potential for substantial increases in factor VIII collection efficiency.
    • Improved availability of FVIII for haemophilia A patients.
    • Prospective consideration of gene-technological approaches for future FVIII production.

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