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Related Experiment Video

Updated: May 4, 2026

Imaging of Intracellular ATP in Organotypic Tissue Slices of the Mouse Brain using the FRET-based Sensor ATeam1.03YEMK
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Metabolic differences in hippocampal 'Rett' neurons revealed by ATP imaging.

J Toloe1, R Mollajew2, S Kügler3

  • 1DFG-Centre of Molecular Physiology of the Brain, Institute of Neuro- and Sensory Physiology, Georg-August-University, Göttingen 37073, Germany; DFG-Centre of Molecular Physiology of the Brain, Department of Neurology, Georg-August-University, Göttingen 37073, Germany.

Molecular and Cellular Neurosciences
|January 8, 2014
PubMed
Summary

Researchers imaged adenosine triphosphate (ATP) in mouse brain slices, revealing differences in energy use between brain regions and in a model for Rett syndrome. This study highlights ATP dynamics in neuronal activity and epilepsy.

Keywords:
CalciumCytoplasmic ATPEpilepsyHippocampusImagingRett syndrome

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Area of Science:

  • Neuroscience
  • Cellular Metabolism
  • Epilepsy Research

Background:

  • Understanding neuronal energy metabolism, particularly adenosine triphosphate (ATP) handling, is crucial for comprehending neuronal function.
  • Existing methods lack the resolution to detail ATP dynamics in specific neuronal populations within living brain tissue.

Purpose of the Study:

  • To visualize and quantify ATP levels and turnover in distinct hippocampal subregions using a genetically encoded sensor.
  • To investigate the role of ATP dynamics in experimentally induced epileptiform activity and in a mouse model of Rett syndrome (MeCP2(-/y)).

Main Methods:

  • Utilized a genetically encoded ATP sensor (Ateam 1.03) specifically targeted to neurons in organotypic hippocampal slices.
  • Induced epileptiform activity by removing extracellular Mg(2+) and monitored concurrent changes in neuronal ATP levels.
  • Examined ATP handling and neuronal activity in hippocampal slices from wild-type and MeCP2(-/y) mice.

Main Results:

  • Distinct patterns of ATP production and consumption were observed in the dentate gyrus and cornu ammonis (CA) areas.
  • Epileptiform activity induced by Mg(2+) withdrawal led to a significant decline in neuronal ATP levels (from 2-3mM to 1-2mM).
  • MeCP2(-/y) neurons exhibited earlier seizure onset, more frequent spontaneous discharges, higher resting ATP levels, and larger ATP drops during activity compared to controls.

Conclusions:

  • Neuronal ATP levels dynamically change during epileptiform activity, influencing the activation of ATP-sensitive potassium (K-ATP) channels.
  • Altered ATP turnover in MeCP2(-/y) neurons may reflect compensatory mechanisms for maintaining hippocampal function in Rett syndrome.
  • Elevated ATP levels and potential K-ATP channel dysfunction in the Rett hippocampus could contribute to increased epilepsy susceptibility.