Complete genome sequence of invertebrate iridovirus IIV30 isolated from the corn earworm, Helicoverpa zea
Benoît Piégu1, Sébastien Guizard1, Tatsinda Spears2
1UMR INRA-CNRS 7247, PRC, Centre INRA de Nouzilly, 37380 Nouzilly, France.
Abstract:
Members of the family Iridoviridae are animal viruses that infect only invertebrates and poikilothermic vertebrates. The invertebrate iridovirus 30 (IIV30) was originally isolated from a larva of the corn earworm, Helicoverpa zea (order lepidoptera, Family Noctuidae) in western Australia. The IIV30 virions are icosahedral, have a diameter of about 130nm, and contain a dsDNA genome of 198.5kbp with 28.11% in GC content and 177 coding sequences. Here we describe its complete genome sequence and annotate the genes for which we could assign a putative function. This is the sixth genome sequence of an invertebrate iridovirus reported.
Insights
The complete genome sequence of invertebrate iridescent virus 30 (IIV30) from Helicoverpa zea was analyzed. This study provides the sixth genome sequence for an invertebrate iridovirus, aiding in understanding insect virus diversity.
Area of Science:
- Virology
- Genomics
- Insect Pathology
Background:
- Iridoviridae are a family of viruses infecting invertebrates and cold-blooded vertebrates.
- Invertebrate iridescent virus 30 (IIV30) was first isolated from corn earworm (Helicoverpa zea) in Australia.
- IIV30 virions are icosahedral, approximately 130nm in diameter, with a dsDNA genome.
Purpose of the Study:
- To present the complete genome sequence of Invertebrate Iridescent Virus 30 (IIV30).
- To annotate genes within the IIV30 genome and identify putative functions.
- To contribute to the growing database of invertebrate iridovirus genomes.
Main Methods:
- Whole-genome sequencing of IIV30.
- Bioinformatic analysis for gene prediction and annotation.
- Comparative genomics with other invertebrate iridoviruses.
Main Results:
- The complete genome sequence of IIV30 was determined (198.5 kbp dsDNA).
- 177 coding sequences were identified, with putative functions assigned to several genes.
- The GC content of the IIV30 genome was found to be 28.11%.
Conclusions:
- This is the sixth reported genome sequence for an invertebrate iridovirus.
- The annotated genome provides insights into the molecular mechanisms of IIV30.
- Further research can build upon this genomic data to understand IIV30 biology and host interactions.
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