Anion-exchange chromatography of normal and monoclonal serum immunoglobulins

Anion-exchange chromatography was used for separations of the three main immunoglobulin classes in human sera. The material included normal sera as well as sera with monoclonal immunoglobulin components. The Mono Q anion-exchange column was eluted with a stepwise gradient formed by 0.05 and 0.3 M phosphate buffers. No indications of negative effects on the sample components were observed and the protein recovery was high. A fast, easy and reproducible separation of immunoglobulins G, A and M was obtained.