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Variations in bile tolerance among Lactococcus lactis strains derived from different sources.
Shihori Takanashi1, Ai Miura, Koko Abe
1Department of Marine Science and Resources, Nihon University, Fujisawa, Kanagawa, 252-0880, Japan.
Folia Microbiologica
|January 8, 2014
Summary
Marine fish-derived Lactococcus lactis subsp. lactis exhibits superior bile tolerance, a crucial trait for its potential use as a probiotic in aquaculture. This strain shows enhanced survival in various bile acid conditions.
Area of Science:
- Microbiology
- Aquaculture Probiotics
Background:
- Lactococcus lactis subsp. lactis is a candidate probiotic for aquaculture.
- Assessing probiotic properties like bile tolerance is essential for its application.
Purpose of the Study:
- To compare the bile tolerance of Lactococcus lactis subsp. lactis strains from different sources (marine fish, freshwater fish, cheese starter) and a related species.
Main Methods:
- Strains were cultured in deMan-Rogosa-Sharpe (MRS) broth with bile salts/acids, MRS agar with oxgall, and phosphate-buffered saline (PBS) with fish bile.
- Survival and growth were assessed under various bile stress conditions.
Main Results:
- Marine fish-derived L. lactis subsp. lactis (MFL) demonstrated the highest survival rates in the presence of sodium cholate, sodium deoxycholate, sodium taurocholate, and oxgall.
- MFL showed no growth inhibition when exposed to bile from goldfish and puffer fish, unlike other strains.
Conclusions:
- Marine fish-derived Lactococcus lactis subsp. lactis possesses significant bile acid resistance.
- This high bile tolerance makes MFL a promising candidate for probiotic development in aquaculture.
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