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Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
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Application of In-Fusion™ cloning for the parallel construction of E. coli expression vectors
Louise E Bird1, Heather Rada, John Flanagan
1Oxford Protein Production Facility-UK, Oxfordshire, UK.
Methods in Molecular Biology (Clifton, N.J.)
|January 8, 2014
Abstract:
In-Fusion™ cloning is a flexible DNA ligase-independent cloning technology that has wide-ranging uses in molecular biology. In this chapter we describe the protocols used in the OPPF-UK to design and construct expression vectors using In-Fusion™. Our method for small scale expression screening in Escherichia coli of constructs generated by In-Fusion™ is also outlined.
