Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Basic characteristics of human lung mast cell desensitization.

D MacGlashan, L M Lichtenstein

Journal of Immunology (Baltimore, Md. : 1950)

|July 15, 1987

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The relationship between total cellular content of CD203c and cell surface expression on human basophils.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2015

Same author

Regulation of IgE-mediated signalling in human basophils by CD32b and its role in Syk down-regulation: basic mechanisms in allergic disease.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2014

Same author

Subthreshold desensitization of human basophils re-capitulates the loss of Syk and FcεRI expression characterized by other methods of desensitization.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2012

Same author

Expression of CD203c and CD63 in human basophils: relationship to differential regulation of piecemeal and anaphylactic degranulation processes.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2010

Same author

IgE-regulated loss, not IgE-regulated synthesis, controls expression of FcepsilonRI in human basophils.

Journal of leukocyte biology·2001

Same author

Signaling cascades: escape from kinetic proofreading.

Proceedings of the National Academy of Sciences of the United States of America·2001

Human mast cells and basophils show similar desensitization mechanisms, with mast cells acting faster. Desensitization inhibits calcium influx, crucial for mediator release, but is unaffected by diisopropylfluorophosphate in mast cells.

Area of Science:

Immunology
Cell Biology

Background:

Mast cells and basophils are key immune cells involved in allergic responses.
Desensitization is a process where cells become less responsive to stimuli after repeated exposure.

Purpose of the Study:

To compare the desensitization processes of human lung mast cells and basophils.
To investigate the biochemical mechanisms underlying mast cell desensitization.

Main Methods:

Human lung mast cells and basophils were stimulated to induce desensitization.
Mediator release (histamine, PGD2, LTC4) and calcium uptake were measured.
The effect of diisopropylfluorophosphate on desensitization was assessed.

Main Results:

Mast cell desensitization kinetics were approximately 3 times faster than basophils.

Related Experiment Videos

Both cell types exhibited similar relationships between mediator release and desensitization.

Desensitization inhibited calcium influx in mast cells but was not affected by diisopropylfluorophosphate, unlike basophils.

Conclusions:

Human mast cells and basophils share similar desensitization biochemistries, with mast cells responding more rapidly.
Mast cell desensitization involves the inhibition of calcium influx, a critical step for mediator release.
A key difference lies in the insensitivity of mast cell desensitization to diisopropylfluorophosphate.