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Expression and anti-apoptotic function of TRAF4 in human breast cancer MCF-7 cells

Xiaoli Zhang1, Zhifeng Wen2, Xiaoyi Mi3

  • 1Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences of China Medical University, Shenyang, Liaoning 110001, P.R. China ; Department of Assisted Reproduction, Shenyang Women's and Children's Hospital, Shenyang, Liaoning 110001, P.R. China.

Oncology Letters
|January 8, 2014
PubMed

Insights

Tumor necrosis factor (TNF) receptor-associated factor 4 (TRAF4) plays a role in breast cancer cell growth. TRAF4 depletion inhibits proliferation and promotes apoptosis in TNF-α-treated breast cancer cells.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Tumor necrosis factor (TNF) receptor-associated factor 4 (TRAF4) is amplified and overexpressed in breast carcinoma.
  • TRAF4's role in breast cancer progression and apoptosis requires further investigation.

Purpose of the Study:

  • To investigate the expression and anti-apoptotic function of TRAF4 in human breast cancer MCF-7 cells.
  • To determine the effect of TRAF4 on cell proliferation and apoptosis induced by TNF-α.

Main Methods:

  • Immunofluorescence staining and western blotting to detect TRAF4 localization and expression.
  • Small interfering RNA (siRNA) to deplete TRAF4 expression in MCF-7 cells.
  • Cell proliferation assays, western blotting for nuclear factor-κB (NF-κB), and cell cycle analysis.

Main Results:

  • TRAF4 expression is higher in breast cancer cells (MCF-7, MDA-MB-231) than in normal breast cells (MCF-10A).
  • TRAF4 is localized in both the cytoplasm and nuclei of MCF-7 cells.
  • TRAF4 depletion suppressed cell proliferation, reduced nuclear NF-κB expression, and increased early apoptosis in TNF-α-treated MCF-7 cells.
  • TRAF4 knockdown led to an increase in G1 phase cells and a decrease in S phase cells.

Conclusions:

  • TRAF4 exhibits anti-apoptotic effects in TNF-α-induced apoptosis in MCF-7 breast cancer cells.
  • TRAF4 contributes to the proliferation of breast cancer cells.
  • Targeting TRAF4 may represent a therapeutic strategy for breast cancer.
Keywords:
TRAF4apoptosisbreast cancersiRNA

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