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Related Experiment Video

Updated: May 4, 2026

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

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Published on: June 23, 2022

Microfluidic immunomagnetic cell separation using integrated permanent micromagnets.

O Osman¹, S Toru¹, F Dumas-Bouchiat²

¹Ampère Laboratory, Ecole Centrale Lyon, CNRS, UMR 5005, 69134 Ecully, France.

Biomicrofluidics

|January 8, 2014

Summary

This summary is machine-generated.

This study presents a microfluidic device for magnetic cell sorting. Labeled Jurkat cells were trapped, while unlabeled HEK 293 cells were eluted, achieving efficient cell separation.

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Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Published on: July 10, 2016

Area of Science:

Biotechnology
Microfluidics
Cell Separation

Background:

Cell sorting is crucial for biological research and diagnostics.
Existing methods for cell separation can be complex or inefficient.
Microfluidic devices offer potential for precise and high-throughput cell manipulation.

Purpose of the Study:

To demonstrate a novel microfluidic device for trapping and sorting labeled cells under flow.
To utilize integrated hard magnetic films for magnetic cell separation.
To compare the efficiency of this microfluidic system with commercial cell separation kits.

Main Methods:

A microfluidic device with integrated micro-patterned hard magnetic film was fabricated.
Jurkat cells were labeled with immunomagnetic nanoparticles; HEK 293 cells remained unlabeled.
Cell mixtures were passed through the microfluidic channel, and cell behavior was analyzed under flow.

Main Results:

Labeled Jurkat cells were attracted to magnetic field gradients and selectively trapped.
Unlabeled HEK 293 cells were eluted, allowing for separation from labeled cells.
Flow cytometry confirmed high enrichment of Jurkat cells in the eluate at increased flow rates.

Conclusions:

The developed microfluidic device enables efficient magnetic cell sorting of labeled cells.
This biocompatible and compact system shows promise as an alternative to commercial cell separation kits.
The technique offers a new approach for cell manipulation in microfluidic systems.