Related Experiment Video

Updated: May 4, 2026

Isolation of Murine Embryonic Hemogenic Endothelial Cells
08:56

Isolation of Murine Embryonic Hemogenic Endothelial Cells

Published on: June 17, 2016

12.3K

Differential expression of distinct surface markers in early endothelial progenitor cells and monocyte-derived

Shu-Meng Cheng1, Shing-Jyh Chang2, Tsung-Neng Tsai1

  • 1Division of Cardiology, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan.

Gene Expression
|January 9, 2014
PubMed

Insights

Distinguishing early endothelial progenitor cells (EPCs) from monocytes is crucial for cell therapies. Early EPCs share gene expression profiles most similar to monocyte-derived macrophages (MDMs), suggesting new isolation markers.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Regenerative Medicine

Background:

  • Endothelial progenitor cells (EPCs) are vital for blood vessel formation.
  • Early EPCs exhibit properties similar to monocytes, complicating their isolation.
  • Pure EPC populations are needed for clinical applications like cardiovascular disease treatment.

Purpose of the Study:

  • To compare gene expression profiles of early EPCs with various blood and endothelial cells.
  • To identify novel biomarkers for distinguishing early EPCs from monocytes and macrophages.
  • To understand the molecular basis for early EPC similarity to monocyte-derived macrophages.

Main Methods:

  • Gene expression profiling of early EPCs, peripheral blood mononuclear cells, monocytes, monocyte-derived macrophages (MDMs), mature endothelial cells, late EPCs, and CD133+ stem cells.
  • Analysis of mRNA profiles to determine cell type similarities.
  • Gene Ontology database search to explore functional significance.
  • Identification of novel plasma membrane genes as potential biomarkers.

Main Results:

  • Early EPCs showed the highest mRNA profile similarity to MDMs compared to all other cell types.
  • Novel potential biomarkers were identified: CLEC5A (high in MDMs), SIGLEC8, and KCNE1 (abundant in early EPCs).
  • Detailed expression profiles and functional modules were elucidated.

Conclusions:

  • Early EPCs molecularly resemble MDMs more than other cell types.
  • Identified genes like SIGLEC8 and KCNE1 may serve as novel biomarkers for purifying early EPCs.
  • These findings can advance cell isolation techniques for therapeutic applications in angiogenesis-related diseases.

More Related Videos

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro09:16

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro

Published on: November 26, 2018

6.9K
Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery08:43

Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery

Published on: February 14, 2017

10.5K

Related Concept Videos

Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
3.1K