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Updated: May 4, 2026

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
Published on: April 11, 2014
Cellular functions of ascorbic acid: a means to determine vitamin C requirements
M Levine1, K R Dhariwal, P W Washko
1Laboratory of Cell Biology and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Abstract:
Optimal ascorbic acid (vitamin C) requirements in humans are unknown. In situ kinetics is a biochemical approach to determine requirements for vitamin C and other vitamins. In situ kinetics requires that cellular functions of ascorbic acid are characterized. Vitamin-C-dependent cellular reactions are directly related to vitamin C concentrations inside and outside cells. By coupling intracellular and extracellular functions of ascorbic acid to vitamin concentration, in situ kinetics provides a novel approach to determining vitamin C requirements.
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