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Identification of Bifidobacterium spp. using hsp60 PCR-RFLP analysis: an update
Verena Stenico1, Samanta Michelini1, Monica Modesto1
1Department of Agricultural Sciences, University of Bologna, Viale Fanin 42, 40127 Bologna, Italy.
Abstract:
A PCR-RFLP technique has been applied on 13 species of Bifidobacterium in order to update a previous study carried out by Baffoni et al. This method is based on the restriction endonuclease activity of HaeIII on the PCR-amplified hsp60 partial gene sequence, and allows a rapid and efficient identification of Bifidobacterium spp. strains at species and subspecies level.
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