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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Updated: Feb 28, 2026

Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH

Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH

Published on: September 20, 2020

DNA microarray-based gene expression profiling of estrogenic chemicals.

Ryoiti Kiyama¹, Yun Zhu

¹Signaling Molecules Research Group, Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan, kiyama.r@aist.go.jp.

Cellular and Molecular Life Sciences : CMLS

|January 9, 2014

Summary

This summary is machine-generated.

DNA microarray gene expression profiling identifies silent estrogens, a novel class of estrogenic chemicals. This technology offers a promising approach for detecting and evaluating diverse estrogenic compounds.

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Area of Science:

Environmental Science
Toxicology
Molecular Biology

Background:

Estrogenic chemicals pose risks to environmental and human health.
Accurate detection and evaluation methods for estrogenic compounds are crucial.

Purpose of the Study:

To review DNA microarray applications for estrogenic chemical detection.
To highlight the identification of silent estrogens using this technology.

Main Methods:

Overview of estrogenic chemicals (natural/industrial estrogens, phytoestrogens).
Gene expression profiling using focused microarrays with estrogen-responsive genes.
Analysis of chemical effects on gene expression patterns.

Main Results:

Comprehensive lists of natural and industrial estrogenic chemicals provided.
Gene expression profiles of various chemicals were summarized.
Silent estrogens identified exclusively via DNA microarray assay, showing estrogenic gene effects without growth alteration.

Conclusions:

DNA microarray assays are effective for identifying novel estrogenic chemical classes like silent estrogens.
The technology shows potential for commercialization and broader applications in chemical safety assessment.
Quality control and future research directions for microarray assays are discussed.