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Related Experiment Video

Updated: May 4, 2026

Author Spotlight: Development of an Enhanced Protocol for Rapid and Accurate Isolation of Campylobacter from Food Products

Author Spotlight: Development of an Enhanced Protocol for Rapid and Accurate Isolation of Campylobacter from Food Products

Published on: February 23, 2024

Isolation of Campylobacter from human stool samples.

S M Salim, J Mandal¹, S C Parija

¹Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India.

Indian Journal of Medical Microbiology

|January 9, 2014

Summary

This summary is machine-generated.

Campylobacter jejuni, a common cause of diarrhea, can be effectively isolated from stool samples using standard laboratory media and a candle jar. This method proved as sensitive as polymerase chain reaction (PCR) for detection.

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Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics MSPP

Published on: October 30, 2016

Area of Science:

Microbiology
Clinical Diagnostics
Infectious Diseases

Background:

Campylobacter is a significant, often undetected, cause of diarrhea in children under five.
Standard isolation methods for Campylobacter are difficult, expensive, and time-consuming.

Purpose of the Study:

To evaluate the efficacy of isolating Campylobacter from stool specimens using routine blood-containing media and a candle jar for microaerophilic atmosphere generation.
To compare this method with standard laboratory techniques and polymerase chain reaction (PCR).

Main Methods:

A descriptive study involving 50 stool samples.
Inoculation onto selective and non-selective media, with and without 0.45 μm membrane filtration.
Incubation under microaerophilic conditions using Anoxomat and candle jars at 37°C and 42°C.
Confirmation by phenotypic tests and simplex PCR targeting 16S ribosomal DNA.

Main Results:

Campylobacter was isolated from 5 out of 50 stool samples using both Anoxomat and candle jar methods.
No significant difference was observed between selective and non-selective media or incubation temperatures.
All isolates were confirmed as Campylobacter jejuni phenotypically and genotypically.
PCR results correlated with culture findings.

Conclusions:

Culture isolation of Campylobacter using routine media and a candle jar is a viable and sensitive method.
This approach is comparable in sensitivity to PCR for Campylobacter detection.