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Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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MALDI-TOF Mass Spectrometry

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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Mass Analyzers: Overview01:13

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The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...
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A Microfluidic Chip for ICPMS Sample Introduction
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Advances in coupling microfluidic chips to mass spectrometry.

Xiaojun Feng1, Bi-Feng Liu1, Jianjun Li2

  • 1Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics-Hubei Bioinformatics and Molecular Imaging Key Laboratory, Systems Biology Theme, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.

Mass Spectrometry Reviews
|January 9, 2014
PubMed
Summary

Microfluidic chip-mass spectrometry (Chip-MS) enhances analytical performance. This review covers Chip-MS innovations in fabrication, ionization techniques, automation, and applications in proteomics, metabolomics, and clinical diagnostics.

Keywords:
ESIMALDImass spectrometrymicrofluidic chip

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Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Materials Science

Background:

  • Microfluidic technology offers advantages like low sample use, faster analysis, and high throughput.
  • Coupling microfluidic chips with mass spectrometry (Chip-MS) significantly boosts analytical performance and application scope.

Purpose of the Study:

  • To review advancements in Chip-MS over the last decade.
  • To cover innovations in microchip fabrication and integration with mass spectrometry.

Main Methods:

  • Review of microchip fabrication techniques.
  • Integration of microfluidic chips with electrospray ionization-mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS).
  • Documentation of integrated microfluidic systems for automated mass spectrometry analysis.

Main Results:

  • Innovations in microchip fabrication for enhanced performance.
  • Successful coupling of microfluidic chips with ESI-MS and MALDI-MS.
  • Development of automated Chip-MS systems.

Conclusions:

  • Chip-MS has advanced significantly, improving MS-based analyses.
  • Chip-MS shows broad applicability in proteomics, metabolomics, cell analysis, and clinical diagnostics.
  • Future developments focus on integrated systems for automated MS analysis.