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Related Experiment Video

Updated: May 4, 2026

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Variability in contrast agent uptake by different but similar stem cell types.

Ashwini Ketkar-Atre1, Tom Struys2, Stefaan J Soenen3

  • 1Biomedical MRI/MoSAIC, Department of Imaging and Pathology, Biomedical Sciences Group, Katholieke Universiteit Leuven, Leuven, Belgium.

International Journal of Nanomedicine
|January 9, 2014
PubMed
Summary

Cell size and proliferation rate significantly impact nanoparticle uptake and toxicity in regenerative medicine. Understanding these cellular parameters is crucial for effective cell tracking and labeling studies using magnetic resonance imaging contrast agents.

Keywords:
MR contrast agentscell labelingiron oxidemagnetic resonance imagingmesenchymal stem cellsmultipotent adult progenitor cellsnanoparticlestransmission electron microscopy

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Area of Science:

  • Regenerative Medicine
  • Biomedical Imaging
  • Nanotechnology

Background:

  • Tracking transplanted cells is vital for regenerative medicine.
  • Pre-labeling cells with magnetic resonance imaging (MRI) contrast agents is a common method.
  • Previous studies focused on nanoparticle (NP)-specific parameters for cell labeling.

Purpose of the Study:

  • To investigate the impact of cellular characteristics on nanoparticle uptake and toxicity.
  • To compare labeling efficiency and effects of different nanoparticles (Endorem®, magnetoliposomes, citrate-coated C-200) in mesenchymal stem cells (MSCs) and multipotent adult progenitor cells (MAPCs).

Main Methods:

  • Utilized three distinct nanoparticle types with varying surface coatings and sizes.
  • Labeled mesenchymal stem cells (MSCs) and multipotent adult progenitor cells (MAPCs).
  • Employed high-resolution electron microscopy for quantitative analysis of iron uptake and intracellular concentrations.

Main Results:

  • Cellular proliferative and morphological characteristics (size, proliferation rate) were key determinants of iron fate, intracellular concentration, and cytotoxicity.
  • A strong correlation was observed between cell volume/surface area, nanoparticle uptake, and cytotoxicity.
  • Reversed trends in uptake and toxicity were noted when considering intracellular concentrations versus absolute amounts.

Conclusions:

  • Cellular parameters like size and proliferation rate are more critical than NP-specific factors in comparative cell-labeling studies.
  • Future research should prioritize these cellular characteristics for optimizing cell tracking and regenerative medicine applications.