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Carbon source dependent promoters in yeasts.

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Summary

This review explores yeast promoters for recombinant protein production. It focuses on carbon source-dependent promoters, particularly those activated upon glucose depletion, for efficient gene expression.

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Microbiology

Background:

  • Budding yeasts are key hosts for recombinant protein production.
  • Transcriptional regulation is critical for efficient gene expression in yeast.
  • A diverse array of promoters (inducible, derepressed, constitutive) are available for yeast expression systems.

Purpose of the Study:

  • To review carbon source-dependent promoters for protein expression in yeasts.
  • To highlight promoters induced or derepressed by specific carbon sources.
  • To focus on promoters activated after glucose depletion for scalable cultivation.

Main Methods:

  • Literature review of yeast promoters.
  • Analysis of carbon source-dependent promoter behavior.
  • Categorization of promoters based on induction/derepression mechanisms.

Main Results:

  • Summarizes a wide range of carbon source-dependent promoters for yeast.
  • Examines common derepressed promoters in Saccharomyces cerevisiae and other yeasts.
  • Describes inducible promoters, including those activated by non-sugar carbon sources.

Conclusions:

  • Carbon source-dependent promoters offer versatile tools for recombinant protein expression in yeast.
  • Promoters activated upon glucose depletion provide efficient and scalable cultivation strategies.
  • Selecting the appropriate promoter is crucial for optimizing protein yields in yeast expression systems.