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Related Experiment Video

Updated: May 4, 2026

Accessing the Cytotoxicity and Cell Response to Biomaterials

Accessing the Cytotoxicity and Cell Response to Biomaterials

Published on: July 8, 2021

Scaffoldless tissue-engineered dental pulp cell constructs for endodontic therapy.

F N Syed-Picard¹, H L Ray, P N Kumta

¹Center for Craniofacial Regeneration, University of Pittsburgh, Pittsburgh, PA 15260, USA.

Journal of Dental Research

|January 10, 2014

Summary

This summary is machine-generated.

Engineered dental pulp cells (DPCs) created scaffoldless 3D tissues for pulp regeneration. These tissues successfully regenerated vital, vascularized pulp-like tissue within tooth root canals in mice, offering promise for endodontic therapy.

Keywords:

calcium phosphate mesenchymal stem cells regeneration root canal tissue engineering tooth

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Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth

Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth

Published on: May 17, 2024

Area of Science:

Biomaterials Science
Regenerative Medicine
Dental Research

Background:

Apical periodontitis often results from bacterial infiltration after endodontic treatment.
Maintaining a vital pulp is crucial for challenging bacterial infiltration.
Pulp regeneration strategies are needed to restore tooth vitality and function.

Purpose of the Study:

To engineer self-assembled, scaffoldless, three-dimensional (3D) tissues from dental pulp cells (DPCs).
To assess the potential of these engineered tissues for pulp regeneration in a tooth root canal system.
To evaluate the vascularization and cellular composition of the regenerated tissue.

Main Methods:

Dental pulp cells (DPCs) were used to create scaffoldless 3D engineered tissues.
These tissues were implanted into human tooth root segments and then subcutaneously in mice.
Histological and immunostaining (CD31, dentin sialoprotein) were performed at three and five months.

Main Results:

Engineered tissues maintained cellular, fibrous tissue within tooth roots, unlike empty controls.
The regenerated tissue within root canals was vascularized (CD31 expression).
Odontoblast-like cells expressing dentin sialoprotein were organized along the root wall.

Conclusions:

3D self-assembled scaffoldless DPC engineered tissues can regenerate vital dental pulp-like tissue.
This approach shows promise for restoring vitality in endodontic therapy.
Scaffoldless tissue engineering offers a viable strategy for pulp regeneration.