Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Waterborne toxicity of several dichlorodiphenyltrichloroethane congeners to Hyalella azteca and its implications for contaminated sediment assessment.

Environmental toxicology and chemistry·2025
Same author

Review of per- and polyfluoroalkyl substances (PFAS) bioaccumulation in earthworms.

Environmental advances·2024
Same author

Dietary Uptake of Highly Hydrophobic Chemicals by Rainbow Trout (Oncorhynchus Mykiss).

Archives of environmental contamination and toxicology·2023
Same author

Review of DDT, DDE, DDD, DDMU and DDMS Toxicity Data for Organisms Used in Estuarine and Marine Sediment Toxicity Tests.

Bulletin of environmental contamination and toxicology·2023
Same author

Biota-Sediment Accumulation Factors for Per- and Polyfluoroalkyl Substances.

Environmental toxicology and chemistry·2022
Same author

Bioaccumulation of Bis-(2-ethylhexyl)-3,4,5,6-tetrabromophthalate and Mono-(2-ethylhexyl)-3,4,5,6-tetrabromophthalate by Lumbriculus variegatus.

Archives of environmental contamination and toxicology·2021

Related Experiment Video

Updated: May 4, 2026

A Lipid Extraction and Analysis Method for Characterizing Soil Microbes in Experiments with Many Samples
17:39

A Lipid Extraction and Analysis Method for Characterizing Soil Microbes in Experiments with Many Samples

Published on: July 16, 2017

22.4K

Evaluation of micro-colorimetric lipid determination method with samples prepared using sonication and accelerated

Nanditha Billa1, Dylan Hubin-Barrows1, Tylor Lahren1

  • 1Mid-Continent Ecology Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency, 6201 Congdon Boulevard, Duluth, MN 55804, USA.

Talanta
|January 10, 2014
PubMed
Summary

Accelerated solvent extraction interferes with micro-colorimetric lipid determination, causing inaccurate results. Sonic extraction, however, is a reliable method for lipid analysis without bias.

Keywords:
Accelerated solvent extractionLipid measurementMicro-colorimetric

More Related Videos

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
09:26

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

Published on: November 17, 2011

15.7K
Phenol Red Thread-based Sampling Procedure for Untargeted Tear Fluid Lipidomics in Biomarker Discovery
08:16

Phenol Red Thread-based Sampling Procedure for Untargeted Tear Fluid Lipidomics in Biomarker Discovery

Published on: December 12, 2025

416

Related Experiment Videos

Last Updated: May 4, 2026

A Lipid Extraction and Analysis Method for Characterizing Soil Microbes in Experiments with Many Samples
17:39

A Lipid Extraction and Analysis Method for Characterizing Soil Microbes in Experiments with Many Samples

Published on: July 16, 2017

22.4K
Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
09:26

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

Published on: November 17, 2011

15.7K
Phenol Red Thread-based Sampling Procedure for Untargeted Tear Fluid Lipidomics in Biomarker Discovery
08:16

Phenol Red Thread-based Sampling Procedure for Untargeted Tear Fluid Lipidomics in Biomarker Discovery

Published on: December 12, 2025

416

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Laboratory Science

Background:

  • Accurate lipid determination is crucial in various scientific fields.
  • Micro-colorimetric methods offer a sensitive approach for quantifying lipids.
  • Validation of extraction techniques is essential for reliable results.

Purpose of the Study:

  • To evaluate two common laboratory extraction techniques for lipid determination.
  • To assess the compatibility of accelerated solvent extraction and sonic extraction with a micro-colorimetric lipid assay.
  • To identify potential interferences and biases in lipid quantification methods.

Main Methods:

  • Comparative analysis of accelerated solvent extraction (ASE) and sonic extraction.
  • Application of the Van Handel micro-colorimetric lipid determination method.
  • Bias assessment using standard additions and Youden Blank measurements at α=1%.

Main Results:

  • ASE with chloroform:methanol solvent showed significant proportional bias in 28/30 samples and constant bias in 1/30.
  • Sonic extraction demonstrated no significant proportional bias (0/6 samples) and only minor constant bias (1/6 samples).
  • The ASE method creates an interference with the colorimetric assay, leading to inaccurate lipid measurements.

Conclusions:

  • Accelerated solvent extraction using chloroform:methanol is incompatible with the micro-colorimetric lipid assay due to significant interference.
  • Sonic extraction is a suitable and unbiased method for routine lipid determination with the evaluated colorimetric assay.
  • Failure to account for ASE-induced bias results in inaccurate lipid quantification.