[Immunobiological analysis of isolated subunits of influenza virus hemagglutinin]

The immunogenic properties of isolated subunits of influenza A/FM/1/47 (H1N1) virus hemagglutinin were studied. The antihemagglutinating serum was tested by the CFT, HI, competitive solid-phase radioimmunoassay, and biological neutralization test. The antiserum was found to contain populations of antibodies to the three main antigenic determinants of hemagglutinin identical to the determinants of the intact virion, as well as antibodies to the antigenic components of host cells. The antiserum to hemagglutinin as well as monospecific antibodies to individual antigenic determinants of hemagglutinin exhibited a high virus-neutralizing activity for the homologous virus.