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Capillary Electrophoresis: Applications01:30

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Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
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Differential ion mobility separations in up to 100% helium using microchips.

Alexandre A Shvartsburg1, Yehia M Ibrahim, Richard D Smith

  • 1Biological Sciences Division, Pacific Northwest National Laboratory, P.O. Box 999, Richland, WA, 99352, USA, alexandre.shvartsburg@pnnl.gov.

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Microscopic channel designs in differential ion mobility spectrometry (FAIMS) enable higher helium gas fractions, improving resolution and sensitivity. This advancement facilitates detailed ion mobility studies for structural analysis.

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Area of Science:

  • Analytical Chemistry
  • Physical Chemistry
  • Spectrometry

Background:

  • Differential Ion Mobility Spectrometry (FAIMS) performance is enhanced by helium (He) gas mixtures.
  • Electrical breakdown limits helium fractions to 50%-75% in conventional FAIMS analyzers.
  • Paschen's law indicates higher breakdown fields at shorter distances, relevant for microchannel devices.

Purpose of the Study:

  • To investigate the use of higher helium fractions in microchannel FAIMS.
  • To determine the impact of increased helium fractions on FAIMS resolution and sensitivity.
  • To enable high-field ion mobility measurements in pure helium for theoretical modeling.

Main Methods:

  • Utilized microchannel chip-based FAIMS analyzers with varying helium fractions up to 100%.
  • Measured ion mobility as a function of electric field strength (E) in helium-rich environments.
  • Compared performance metrics (resolution, sensitivity) with conventional FAIMS systems.

Main Results:

  • Microchannel FAIMS successfully employed helium fractions up to 100%, exceeding previous limits.
  • Higher helium fractions significantly improved the resolution and resolution/sensitivity balance.
  • Optimal helium fraction determined to be approximately 80%, aligning with theoretical predictions.

Conclusions:

  • Microchannel FAIMS technology overcomes previous helium fraction limitations.
  • Enables direct measurement of ion mobility dependence on electric field in pure helium.
  • Facilitates quantitative modeling for a priori extraction of ion structural information.