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Updated: May 4, 2026
Detection of microRNA Expression in Peritoneal Membrane of Rats Using Quantitative Real-time PCR
Published on: June 27, 2017
[Micro RNA-155 expression in hemodialysis PBMC]
Introduction:
MicroRNAs (miR) are fragments of non-coding RNA acting at post-transcriptional level, which modulate gene expression, and play a key role in several pathophysiological pathways. The aim of this study is to analyze the expression of miR-155 in basal Peripheral Blood Mononuclear Cells (PBMC) of chronic hemodialysis (HD) patients, before and after standard 4 -hour sessions, and after PHA stimulation compared with PBMC from healthy subjects.
Methods:
miR-155 was isolated from chronic HD patients' PBMC and from PBMC derived from healthy subjects. Expression levels were quantified with Real-Time PCR; PCR reactions were performed using a specific thermocycler and cycle threshold levels were calculated using dedicated software. Blood samples were taken from HD patients after the long inter-dialytic interval. Data were expressed as MSE and statistical differences were calculated with t-test.
Results:
Relative quantity (RQ) of pre-dialysis MiR-155 was 3.770.62 times higher than the control group (P=0,003). There was no significant difference before and after hemodialysis sessions. Pre-dialysis mir-155 RQ in PHA PBMC was 1.790.59 times higher than non stimulated PBMC (P=0.019).
Conclusions:
these preliminary data show a significant miR-155 up-regulation of HD PBMC when compared to PBMC from healthy individuals. An additional up-regulation was observed in HD PHA PBMC. Similar miR-155 expression was found in HD PBMC comparing pre and post-hemodialysis sessions.
Insights
MicroRNA-155 (miR-155) is significantly upregulated in peripheral blood mononuclear cells (PBMC) of hemodialysis patients compared to healthy individuals. This upregulation is also observed after PHA stimulation, but not significantly altered by hemodialysis sessions.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Context:
- MicroRNAs (miRNAs) are crucial post-transcriptional regulators of gene expression involved in various pathophysiological processes.
- Chronic kidney disease patients undergoing hemodialysis exhibit altered cellular functions and inflammatory states.
Purpose:
- To investigate the expression levels of miR-155 in peripheral blood mononuclear cells (PBMC) from chronic hemodialysis (HD) patients.
- To compare miR-155 expression in HD patients' PBMC before and after hemodialysis sessions.
- To assess the effect of phytohemagglutinin (PHA) stimulation on miR-155 expression in HD patients' PBMC.
Summary:
- Real-Time PCR analysis revealed significantly higher miR-155 relative quantity (RQ) in pre-dialysis PBMC of HD patients compared to healthy controls (3.77-fold increase, P=0.003).
- PHA stimulation further increased miR-155 RQ in HD patients' PBMC (1.79-fold increase, P=0.019) compared to non-stimulated PBMC.
- No significant difference in miR-155 expression was observed in HD patients' PBMC before and after hemodialysis sessions.
Impact:
- These findings indicate a significant upregulation of miR-155 in HD patients' PBMC, suggesting its potential role in the pathophysiology of chronic kidney disease.
- The observed miR-155 upregulation, particularly after PHA stimulation, may point towards an altered immune response in HD patients.
- Further research is warranted to elucidate the functional consequences of miR-155 dysregulation in hemodialysis patients.
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