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A new concept in hybridization: Bromelain enzyme for deproteinizing dentin before application of adhesive system
Raad Niama Dayem1, Mona Adnan Tameesh1
1Professor Dr. Raad Niama Dayem BDS, MSc, PhD Conservative Dentistry, USA.
Objective:
The objective of this study is to assess the deproteinizing effect of bromelain enzyme and compare it with neodymium-doped yttrium aluminum garnet (Nd:YAG) laser and 10% sodium hypochlorite (NaOCl) by using scanning electron microscope (SEM) and polarized microscope.
Materials And Methods:
A total of 60 extracted human upper premolars were selected to be given standardized buccal and lingual class V cavities. The teeth were divided into three groups each one consisted of 20 teeth. Thirty teeth were recruited for SEM study and the other 30 for polarized microscope. Group 1: Teeth were deproteinized with Nd:YAG laser, Group 2: Teeth were deproteinized with bromelain enzyme and Group 3: Teeth were deproteinized with 10% NaOCl.
Results And Conclusions:
Application of bromelain enzyme has led to removing collagen network and significantly decreased the global leakage scores of the adhesive system.
