Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ophthalmic Drug Delivery Systems01:23

Ophthalmic Drug Delivery Systems

283
Ophthalmic drug delivery faces major limitations due to poor absorption across the corneal membrane. This process is primarily driven by diffusion and is influenced by two main factors: the physicochemical properties of the drug and tear drainage. Most ophthalmic drugs, such as pilocarpine, epinephrine, atropine, and local anesthetics, are weak bases. They are typically formulated at an acidic pH to enhance chemical stability. However, this leads to high ionization, reducing their ability to...
283

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-speed liquid switching and on-chip force sensing reveal the transient mechanical response of MscL in <i>Synechocystis</i> sp. PCC 6803.

Lab on a chip·2026
Same author

Deployable and Stiffness-Variable Miniature Actuator with Water Circulation Channel and Shape Memory Polymer.

Soft robotics·2026
Same author

An ancestral glutamate receptor mediates cell volume regulation during high K<sup>+</sup> stress in cyanobacteria.

The Journal of biological chemistry·2026
Same author

Dual vision-equipped microfluidic chip for spatiotemporal sequential pick-and-place of oocytes.

Lab on a chip·2026
Same author

Traction Cybernetic Avatar System for Colon ESD.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2025
Same author

EVALUATION OF HAND TREMOR SUPPRESSION USING A PASSIVE SURGICAL SUPPORT ROBOT IN SIMULATED RETINAL VASCULAR CANNULATION.

Retina (Philadelphia, Pa.)·2025
Same journal

Parallelized contactless microfluidic dispenser with superhydrophobic nozzles for scalable combinatorial screening.

Biomicrofluidics·2026
Same journal

Time resolved luminescence of millisecond lifetime dyes in droplet microfluidic systems.

Biomicrofluidics·2026
Same journal

Emerging trends in functional molecularly imprinted polymers for electrochemical detection of biomarkers.

Biomicrofluidics·2025
Same journal

Deep learning assisted mechanotyping of individual cells through repeated deformations and relaxations in undulating channels.

Biomicrofluidics·2025
Same journal

<i>Giardia</i> purification from fecal samples using rigid spiral inertial microfluidics.

Biomicrofluidics·2025
Same journal

Point of care sepsis diagnosis: Exploring microfluidic techniques for sample preparation, biomarker isolation, and detection.

Biomicrofluidics·2025
See all related articles

Related Experiment Video

Updated: May 4, 2026

Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development
09:22

Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development

Published on: February 9, 2015

24.4K

Accurate dispensing system for single oocytes using air ejection.

Lin Feng1, Yiling Sun1, Chisato Ohsumi1

  • 1Department of Micro-Nano Systems Engineering, Graduate School of Engineering, Nagoya University, 1 Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.

Biomicrofluidics
|January 10, 2014
PubMed
Summary
This summary is machine-generated.

This study introduces a microfluidic system for 100% accurate single-oocyte dispensing. The innovative design ensures high oocyte survival rates, crucial for assisted reproduction technologies.

More Related Videos

Xenopus Oocytes: Optimized Methods for Microinjection, Removal of Follicular Cell Layers, and Fast Solution Changes in Electrophysiological Experiments
07:24

Xenopus Oocytes: Optimized Methods for Microinjection, Removal of Follicular Cell Layers, and Fast Solution Changes in Electrophysiological Experiments

Published on: December 31, 2016

20.0K
Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing
09:16

Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing

Published on: October 11, 2015

11.0K

Related Experiment Videos

Last Updated: May 4, 2026

Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development
09:22

Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development

Published on: February 9, 2015

24.4K
Xenopus Oocytes: Optimized Methods for Microinjection, Removal of Follicular Cell Layers, and Fast Solution Changes in Electrophysiological Experiments
07:24

Xenopus Oocytes: Optimized Methods for Microinjection, Removal of Follicular Cell Layers, and Fast Solution Changes in Electrophysiological Experiments

Published on: December 31, 2016

20.0K
Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing
09:16

Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing

Published on: October 11, 2015

11.0K

Area of Science:

  • Biotechnology
  • Microfluidics
  • Reproductive Biology

Background:

  • Accurate single-oocyte dispensing is critical for assisted reproductive technologies.
  • Existing methods face challenges with success and viability rates.
  • Microfluidic systems offer potential for precise cell manipulation.

Purpose of the Study:

  • To develop and validate a novel microfluidic system for high-success, high-viability single-oocyte dispensing.
  • To investigate the impact of the dispensing process on oocyte survival rates.
  • To assess the system's potential for applications like two-dimensional micropatterning.

Main Methods:

  • Utilized a microfluidic chip with capacitance sensors for oocyte detection.
  • Engineered a buffer zone with micro-pillars to decelerate oocytes and reduce hydraulic pressure.
  • Implemented an airflow ejection mechanism for precise oocyte transfer to a culture array.

Main Results:

  • Achieved a 100% success rate for single-oocyte dispensing.
  • Maintained an approximate 70% oocyte survival rate, consistent with pre-dispensing rates.
  • Demonstrated the system's high operational speed and potential for micropatterning.

Conclusions:

  • The developed microfluidic system significantly enhances single-oocyte dispensing accuracy without compromising oocyte viability.
  • This technology offers a robust solution for oocyte handling in reproductive biology and beyond.
  • The system's efficiency and versatility support its application in advanced cell manipulation techniques.