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Overview Of Cell Separation And Isolation

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

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Manufacturing and wetting low-cost microfluidic cell separation devices.

Ryan S Pawell¹, David W Inglis², Tracie J Barber¹

¹Department Mechanical and Manufacturing Engineering, University of New South Wales, New South Wales 2052, Australia.

Biomicrofluidics

|January 10, 2014

Summary

We developed a cost-effective thermoplastic fabrication protocol for deterministic lateral displacement (DLD) devices, enabling rapid manufacturing of microfluidic filters for cell separation. This method significantly reduces production time and costs for this promising technology.

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Area of Science:

Microfluidics
Biotechnology
Materials Science

Background:

Deterministic lateral displacement (DLD) is a microfluidic technology for size-based particle separation.
Existing manufacturing methods for DLD devices lack cost-effectiveness.
There is a need for efficient and affordable fabrication protocols for DLD applications in cell separation and enrichment.

Purpose of the Study:

To develop a simple, robust, and cost-effective protocol for fabricating thermoplastic DLD devices.
To utilize regulatory-approved and biocompatible materials for DLD device manufacturing.
To assess the accuracy and precision of the developed fabrication method.

Main Methods:

Thermoplastic fabrication of DLD devices using biocompatible materials.
Volumetric flow rate testing of the fabricated DLD devices.
Optical profilometry and image analysis for assessing manufacturing accuracy and precision.

Main Results:

The developed protocol resulted in a standalone DLD device manufactured in under 1 hour.
The device achieved volumetric flow rates of 660 μl/min.
Manufacturing accuracy showed an average replicated post height difference of 0.48% and an average replicated array pitch difference of 1.1% compared to the master mold.

Conclusions:

A cost-effective and rapid protocol for thermoplastic DLD device fabrication was successfully developed.
The fabricated DLD devices demonstrate high accuracy and precision, suitable for cell separation applications.
This manufacturing advancement lowers the barrier for implementing DLD technology in various biological and medical fields.