Optimisation of phenolics recovery from Vitex agnus-castus Linn. leaves by high-pressure and temperature extraction
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Updated: May 4, 2026

Employing Pressurized Hot Water Extraction PHWE to Explore Natural Products Chemistry in the Undergraduate Laboratory
Published on: November 7, 2018
Mohammed Lataoui1, Mongi Seffen, Bahar Aliakbarian
1a Laboratory of Energy and Materials, High School of Sciences and Technology, Sousse University , Rue Lamine Abassi 4011, Hammam, Sousse , Tunisia.
Abstract:
To optimise recovery of phenolics from Vitex agnus-castus Linn., a non-conventional high-pressure (2-24 bar) and temperature (100-180°C) extraction method was used under nitrogen atmosphere with methanol as a solvent. Optimal temperature was between 100 and 140°C, and optimal extraction time was about one half that of conventional solid/liquid extraction at room temperature. Final yields of total polyphenols, total flavonoids, o-diphenols and anthocyanins extraction were 2.0, 3.0, 2.5 and 11-fold those obtained by conventional extraction.
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