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Published on: October 1, 2007
Anti-ovalbumin antibody binds to 3T3 cells and induces DNA synthesis
Abstract:
Rabbit anti-ovalbumin antibodies were found to bind to Swiss NIH 3T3 fibroblasts by indirect immunofluorescence, and to induce DNA synthesis when these cells were arrested in G0 phase of their replicating cycle (G0-S activity). The proof that these effects were related to the ovalbumin paratopes is based on the following arguments. Antibodies prepared by affinity chromatography from rabbits immunized in the presence or absence of complete Freund adjuvant, possessed the same specific activity. Immunoglobulins from ovalbumin-immunized rabbits were isolated on a protein A Sepharose column and then fractionated on insoluble ovalbumin; the retained fraction displayed G0-S activity and showed indirect immunofluorescence, whilst the non-retained fraction was inactive. Fluorescence was specifically quenched by ovalbumin and the G0-S activity recovered in the ovalbumin immune precipitate. (Fab')2 fragments had the same effects as the native immunoglobulins, but presented a moderate decrease in specific activity.
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