[Genetics of bovine lactate dehydrogenase isoenzymes]

Instead of 5 usual isoenzyme bands of lactate dehydrogenase 15 bands were found in bloodserum of 75 cattle from different breeds using a special separation technique of polyacrylamid disc electrophoresis. -Because LDH-5 resolved in a total of 5 bands it was suggested that the 5 LDH isoenzymes are formed by 3 polypeptids BAA' which are under separate genetic control. With a simple genetical test it was demonstrated for the dairy herd examined (p < 0,001) that the genes controlling B, A and A' are placed on three independent loci and that there is no isoenzyme polymorphism. In 2 of 15 additionally checked waterbuffalos no resolving into 15bands was registrated, that means that a LDH-polymorphism probably may occur in buffalos.