Elucidating the genetic basis for Escherichia coli defense against silver toxicity using mutant arrays

Bacterial adaptation and defense mechanisms against silver are poorly understood at the genetic level. A library of Escherichia coli gene-deletion mutants was used to show that clones lacking sodB (coding for oxidative stress protection), lon (protein damage repair), or cusR (metal efflux pump) are quite sensitive to silver (with 7.3 ± 9.1%, 5.3 ± 1.8%, and 0.4 ± 0.1% of cells surviving, respectively, compared with 90.1 ± 5.4% survival for wild-type E. coli, after 6-h exposure to 8 mg/L AgNO(3)), suggesting the importance of the coded functions as defense mechanisms. Mutants lacking pgaB or wcaD, which code for production of extracellular polymeric substances (EPS), also showed significant (p < 0.05) sensitivity to silver exposure (23.4 ± 16.2% and 23.1 ± 32.6% survival, respectively). Transmission electron microscopy (TEM) with scanning TEM/energy-dispersive X-ray spectroscopy analysis showed accumulation of silver nanoparticles within EPS, suggesting that EPS serve as a protective barrier that also immobilizes dissolved silver as silver nanoparticles.