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A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
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Detecting Concentration of Analytes with DETECHIP: A Molecular Sensing Array.

Hannah Johnke1, Gary Batres1, Mark Wilson1

  • 1Department of Chemistry, Doane College, Crete, USA.

Journal of Sensor Technology
|January 11, 2014
PubMed
Summary

The DETECHIP detection system now quantifies analyte concentrations by analyzing color changes and UV-Vis spectra. This advancement provides linear, high-correlation data for known substances, enhancing drug detection capabilities.

Keywords:
Analyte ConcentrationColorimetric ArraysDrug DetectionRGB AnalysisSensors

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Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Chemical Sensing

Background:

  • DETECHIP® is a sensor-based detection system capable of identifying small molecules, including illicit and over-the-counter drugs.
  • Previous versions of DETECHIP normalized detection to a single analyte concentration.
  • The need for quantitative detection of varying analyte concentrations is critical in forensic and clinical settings.

Purpose of the Study:

  • To expand the capabilities of the DETECHIP® system to quantitatively determine analyte concentrations.
  • To establish a method for detecting concentration differences using colorimetric and spectral changes.
  • To validate the linear relationship between analyte concentration and observed signal changes.

Main Methods:

  • Utilizing DETECHIP® assays with varying concentrations of analytes.
  • Scanning exposed assays using a flatbed scanner to capture color information.
  • Analyzing Red, Green, and Blue (RGB) values from scanned images using ImageJ software.
  • Measuring shifts in UV-Vis spectra to correlate with analyte concentration.

Main Results:

  • Increasing analyte concentrations led to significant differences in RGB color values between control and analyte wells.
  • A linear relationship was observed between analyte concentration and the magnitude of color value differences.
  • Correlation coefficients exceeding 98% were achieved for some analytes, demonstrating high accuracy.
  • UV-Vis spectral shifts also correlated with concentration differences.

Conclusions:

  • The enhanced DETECHIP® assay can accurately detect and quantify concentration differences of known analytes.
  • This quantitative capability significantly expands the utility of DETECHIP® in various analytical applications.
  • The system offers a sensitive and reliable method for concentration determination based on colorimetric and spectral analysis.