[An immunohistochemical study of various breast tissues using CA15-3 (MAb 115D8 and MAb DF3)]

A CA15-3 RIA KIT, composed with two different monoclonal antibodies (115D 8 and DF 3), has been seen to react with the sera of breast cancer patients. Although the subclass of both antibodies is different, the antigen that reacted with them seems to be same, with a range from 300-450 kd. To reveal the reacting pattern of both antibodies, an immunohistochemical study was performed involving various breast tissues. In general, normal and benign breast tumors exhibited an apical stain by 115D8 and an apical and focal cytosol stain by DF 3. Breast carcinomas displayed not only an apical stain but a strong cytosol stain. However, the staining patterns showed little difference.