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Related Experiment Video

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Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy
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Bacterial imaging with photostable upconversion fluorescent nanoparticles.

Li Ching Ong1, Lei Yin Ang2, Sylvie Alonso3

  • 1Graduate School for Integrative Sciences and Engineering, National University of Singapore, Centre for Life Sciences (CeLS), #05-01, 28 Medical Drive, Singapore 117456, Singapore.

Biomaterials
|January 14, 2014
PubMed
Summary

Upconversion nanoparticles (UCNs) offer superior photostability for bacteria labeling compared to traditional methods. This enables prolonged imaging of bacterial dissemination and trafficking in cells.

Keywords:
Bacteria labelingEscherichia coliFluorescence imagingUpconversion nanoparticles

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Area of Science:

  • Biotechnology
  • Nanotechnology
  • Microbiology

Background:

  • Traditional bacteria labeling methods using fluorophores and fluorescent proteins suffer from autofluorescence, photodamage, and photobleaching.
  • These limitations hinder long-term monitoring of bacterial dissemination and infection dynamics.

Purpose of the Study:

  • To develop and evaluate a novel bacteria labeling strategy using upconversion nanoparticles (UCNs).
  • To overcome the limitations of conventional labeling techniques for prolonged bacteria imaging.

Main Methods:

  • Labeling of Escherichia coli with UCNs.
  • Comparative analysis of UCN-labeled bacteria versus green fluorescent protein-expressing bacteria for photostability.
  • Infection studies using dendritic cells to assess UCN signal co-localization and long-term imaging.

Main Results:

  • UCN-labeled bacteria exhibited significantly superior photostability compared to green fluorescent protein-expressing bacteria.
  • UCN signals successfully co-localized with UCN-labeled bacteria in dendritic cells for up to 6 hours post-infection.
  • Demonstrated potential for long-term monitoring of bacterial trafficking using UCN-based imaging.

Conclusions:

  • Upconversion nanoparticles provide a photostable and effective alternative for bacteria labeling.
  • UCN-based imaging facilitates prolonged observation of bacterial dissemination and intracellular trafficking.