Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sample handling techniques when analyzing regulatory peptides.

E Theodorsson-Norheim, A Hemsén, E Brodin

    Life Sciences
    |August 17, 1987
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Simultaneous determination of cholecystokinin, dopamine, glutamate and aspartate in cortex and striatum of the rat using in vivo microdialysis.

    Amino acids·2013
    Same author

    Serum levels of vitamin D are not associated with future risk of venous thromboembolism. The Tromsø Study.

    Thrombosis and haemostasis·2013
    Same author

    Monocyte count and procoagulant functions are associated with risk of venous thromboembolism: the Tromsø study.

    Journal of thrombosis and haemostasis : JTH·2011
    Same author

    The Swedish version of the Haemophilia Activity List.

    Haemophilia : the official journal of the World Federation of Hemophilia·2011
    Same author

    Vascular effects, formation, clearance and release of endothelin peptides in the pig.

    Neurochemistry international·2010
    Same author

    Endogenous thrombin potential (ETP) in plasma from patients with AMI during antithrombotic treatment.

    Thrombosis research·2008

    Proper sample handling, including rapid cooling and freezing, is crucial for preserving neuropeptide Y (NPY) and other neuropeptides. Storage significantly reduces NPY recovery, highlighting the need for optimized collection and preservation methods.

    Area of Science:

    • Biochemistry
    • Neuroscience
    • Analytical Chemistry

    Background:

    • Neuropeptides are vital signaling molecules in the nervous system.
    • Accurate quantification of neuropeptides is essential for research and diagnostics.
    • Degradation and instability pose challenges in neuropeptide analysis.

    Purpose of the Study:

    • To investigate optimal methods for preserving immunoreactive neuropeptides in biological samples.
    • To evaluate the stability of neuropeptides under various storage and extraction conditions.
    • To determine the impact of pre-analytical factors on neuropeptide quantification.

    Main Methods:

    • Blood samples were collected and processed under varying conditions (cooling, centrifugation, inhibitors).
    • Plasma and tissue extracts were stored at different temperatures and for extended periods.

    Related Experiment Videos

  • Radioimmunoassay (RIA) and chromatographic techniques were used to measure neuropeptide concentrations.
  • Extraction methods (acid ethanol, Sep Pak) were assessed for recovery and purity.

    • Main Results:

    • Rapid cooling and centrifugation were more critical than enzyme inhibitors for preserving neuropeptide Y (NPY).
    • Frozen storage (-20°C or -80°C) for nine months reduced NPY recovery by approximately 50%.
    • Substance P degraded rapidly in plasma at 37°C, while neurokinin A and neuropeptide K were more stable.
    • Neuropeptide immunoreactivity in spinal cord extracts showed heterogeneity upon prolonged storage, especially at room temperature.
    • Acid ethanol and Sep Pak extraction yielded high recovery (>90%) of NPY, neuropeptide K, and calcitonin gene-related peptide.

    Conclusions:

    • Pre-analytical variables significantly impact neuropeptide stability and recovery.
    • Optimized sample collection, rapid cooling, and appropriate extraction methods are vital for accurate neuropeptide measurement.
    • Storage conditions, particularly temperature and duration, affect neuropeptide integrity, necessitating careful consideration for long-term biobanking.