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Somatostatin receptor-2 negatively regulates β-adrenergic receptor mediated Ca(2+) dependent signaling pathways in

Rishi K Somvanshi1, Shenglong Zou1, Xiaofan Qiu1

  • 1Faculty of Pharmaceutical Sciences, The University of British Columbia, Vancouver, BC V6T1Z3, Canada.

Biochimica Et Biophysica Acta
|January 14, 2014
PubMed

Insights

Somatostatin receptor 2 (SSTR2) protects the heart by regulating calcium signaling pathways. This receptor modulates beta-adrenergic receptor (β-AR) activity, preventing cardiac hypertrophy and complications.

Area of Science:

  • Cardiology
  • Molecular Biology
  • Pharmacology

Background:

  • Beta-adrenergic receptors (β-ARs) mediate cardiac function but their overactivation can lead to hypertrophy.
  • Intracellular calcium (Ca2+) signaling is critical in cardiac function and hypertrophy.
  • Somatostatin receptor 2 (SSTR2) role in cardiac signaling is not fully understood.

Purpose of the Study:

  • To investigate the role of SSTR2 in modulating β-AR signaling pathways.
  • To determine if SSTR2 activation impacts Ca2+ handling and cardiac hypertrophy.

Main Methods:

  • Utilized H9c2 cells to study receptor interactions and signaling.
  • Administered SSTR2 selective agonists and β-AR agonists (isoproterenol, formoterol).
  • Assessed cAMP formation, protein kinase A (PKA) phosphorylation, protein kinase C (PKC) expression, calcineurin activity, NFAT translocation, and cellular hypertrophy markers.

Main Results:

  • SSTR2 colocalizes with β1AR and β2AR in H9c2 cells.
  • SSTR2 activation inhibited isoproterenol/formoterol-induced cAMP production, PKA phosphorylation, and PKC expression.
  • SSTR2 activation suppressed calcineurin/NFAT signaling and prevented isoproterenol-induced cardiac hypertrophy.

Conclusions:

  • SSTR2 plays a significant role in modulating β-AR mediated signaling pathways.
  • SSTR2 activation demonstrates a protective effect against cardiac hypertrophy by regulating Ca2+ signaling.
  • SSTR2 represents a potential therapeutic target for managing cardiac hypertrophy.
Keywords:
Adrenergic receptorH9c2 cellHypertrophySignalingSomatostatin receptor 2

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