Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

8.2K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
8.2K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

8.1K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
8.1K
Position-effect Variegation02:32

Position-effect Variegation

5.6K
In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
5.6K
Histone Modification02:32

Histone Modification

14.6K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
14.6K
Histone Modification02:32

Histone Modification

4.0K
4.0K
Ribosome Profiling02:24

Ribosome Profiling

3.2K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction: GLYATL1 is associated with metabolic and epigenetic changes and with endocrine resistance in luminal breast cancer.

Clinical epigenetics·2026
Same author

A microRNA generated via lysosomal processing of ribosomal RNA suppresses proinflammatory responses.

Life science alliance·2026
Same author

Activated T cell extracellular vesicle DNA transfer enhances antigen presentation and anti-tumor immunity.

Cancer cell·2026
Same author

GLYATL1 is associated with metabolic and epigenetic changes and with endocrine resistance in luminal breast cancer.

Clinical epigenetics·2026
Same author

p53 regulates the expression of histone modifiers to restrict stemness and maintain differentiated luminal identity in breast cancer.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

The legacy of a gentleman scientist: Pierre Hainaut.

Cell death and differentiation·2025

Related Experiment Video

Updated: May 4, 2026

In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones
11:36

In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones

Published on: July 25, 2019

10.6K

Writing and reading H2B monoubiquitylation.

Gilad Fuchs1, Moshe Oren1

  • 1Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel.

Biochimica Et Biophysica Acta
|January 14, 2014
PubMed
Summary

Histone H2B monoubiquitylation (H2Bub1) by RNF20/40 impacts chromatin structure and function. H2Bub1 readers mediate its effects on transcription and other cellular processes.

Keywords:
H2B monoubiquitylationH2Bub1Histone crosstalkTranscription elongation

More Related Videos

Detection of Protein Ubiquitination
09:00

Detection of Protein Ubiquitination

Published on: August 19, 2009

42.8K
Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates
09:47

Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates

Published on: May 10, 2022

2.4K

Related Experiment Videos

Last Updated: May 4, 2026

In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones
11:36

In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones

Published on: July 25, 2019

10.6K
Detection of Protein Ubiquitination
09:00

Detection of Protein Ubiquitination

Published on: August 19, 2009

42.8K
Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates
09:47

Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates

Published on: May 10, 2022

2.4K

Area of Science:

  • Epigenetics and Molecular Biology
  • Chromatin Biology
  • Gene Regulation

Background:

  • Histone H2B monoubiquitylation (H2Bub1) is a key epigenetic mark.
  • Catalyzed by the RNF20/40 complex, H2Bub1 influences chromatin structure.
  • Its direct effects on nucleosome stability and compaction are known.

Purpose of the Study:

  • To review the current understanding of H2Bub1-specific readers.
  • To elucidate the indirect roles of H2Bub1 in biological processes.
  • To highlight the significance of H2Bub1 readers in gene regulation.

Main Methods:

  • Literature review and synthesis of existing research.
  • Analysis of studies on H2Bub1 reader proteins.
  • Integration of findings on H2Bub1's role in DNA-templated processes.

Main Results:

  • H2Bub1 regulates chromatin through direct biochemical changes.
  • H2Bub1 recruits specific reader proteins to chromatin.
  • These readers mediate H2Bub1's impact on histone crosstalk and transcription.

Conclusions:

  • H2Bub1 readers are crucial mediators of H2Bub1 function.
  • Understanding H2Bub1 readers provides insight into epigenetic regulation.
  • H2Bub1 and its readers play vital roles in diverse biological activities.