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Updated: May 4, 2026

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

Information multiplexing in ptychography.

Darren J Batey¹, Daniel Claus¹, John M Rodenburg²

¹Department of Electrical and Electronic Engineering, University of Sheffield, S1 3JD Sheffield, UK.

Ultramicroscopy

|January 14, 2014

Summary

This summary is machine-generated.

Ptychography (lensless diffractive imaging) can now recover an object's spectral response by reconstructing multiple images. This breakthrough utilizes incoherent modes, previously a challenge, to gain more information and enable information multiplexing across wavelengths.

Keywords:

Biomedical imaging Coherent diffractive imaging Information multiplexing Optical metrology Ptychography

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Area of Science:

Diffractive imaging
Lensless imaging techniques
Computational imaging

Background:

Ptychography is a lensless imaging technique that reconstructs an object's properties from a series of diffraction patterns.
Incoherent modes in illumination have traditionally complicated image reconstruction in diffractive imaging.
Solving the phase problem is crucial for accurate image reconstruction in diffractive imaging.

Purpose of the Study:

To demonstrate that ptychography can recover an object's spectral response by simultaneously reconstructing multiple images.
To show that incoherent modes, previously considered a nuisance, can be leveraged to extract more information.
To enable simultaneous reconstruction of multiple illumination modes.

Main Methods:

Simultaneous reconstruction of multiple images representing the object's response to specific illumination modes.
Independent solution of the phase problem for each illumination mode.
Utilizing incoherent superposition of diffracted waves to recover spectral information.

Main Results:

Successfully recovered the spectral response of an object using ptychography.
Demonstrated that incoherent modes can be effectively managed and utilized for enhanced information retrieval.
Showed the capability to solve for multiple illumination modes simultaneously.

Conclusions:

Ptychography can be extended to recover spectral information by utilizing incoherent modes.
This approach overcomes previous limitations associated with incoherent illumination in diffractive imaging.
The method opens possibilities for information multiplexing in ptychography across various wavelengths (visible, X-ray, electron).