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Efficient and Rapid Isolation of Early-stage Embryos from Arabidopsis thaliana Seeds
Published on: June 7, 2013
Correlation between deoxyribonuclease activity and DNA replication in the embryonic axes of germinating peas (Pisum
1Department of Botany, University College, P.O. Box 78, CF1 1XL, Cardiff, U.K..
Abstract:
Crude chromatin preparations from pea seedlings contain calcium-dependent deoxyribonuclease activity, at least part of which is endonucleolytic. During germination, there is a dramatic increase in chromatin-bound deoxyribonuclease activity in the embryonic axis immediately prior to the onset of DNA replication. Evidence has been obtained for the presence of an inhibitor of deoxyribonuclease activity in chromatin preparations from embryonic axes not undergoing DNA replication.
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