Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Photosynthetic acclimation in a terrestrial CAM bromeliad, Bromelia humilis Jacq.

The New phytologist·2021

Same author

CARBON DIOXIDE AND WATER DEMAND: CRASSULACEAN ACID METABOLISM (CAM), A VERSATILE ECOLOGICAL ADAPTATION EXEMPLIFYING THE NEED FOR INTEGRATION IN ECOPHYSIOLOGICAL WORK.

The New phytologist·2021

Same author

Ecophysiology of xerophytic and halophytic vegetation of a coastal alluvial plain in northern Venezuela: IV. Tillandsia flexuosa Sw. and Schomburgkia humboldtiana Reichb., epiphytic CAM plants.

The New phytologist·2021

Same author

Ecophysiology of xerophytic and halophytic vegetation of a coastal alluvial plain in northern Venezuela: III. Bromelia humilis Jacq., a terrestrial CAM bromeliad.

The New phytologist·2021

Same author

Ecophysiology of xerophytic and halophytic vegetation of a coastal alluvial plain in northern Venezuela: VI. Water relations and gas exchange of mangroves.

The New phytologist·2021

Same author

Ecophysiology of xerophytic and halophytic vegetation of a coastal alluvial plain in northern Venezuela: V. The Batis maritime-Sesuvium portulacastrum vegetation unit.

The New phytologist·2021

Search research articles

Related Experiment Video

Updated: May 4, 2026

High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits

High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits

Published on: September 20, 2016

Active hexose uptake in Lemna gibba G1.

C I Ullrich-Eberius¹, A Novacky, U Lüttge

¹Institut für Botanik der Technischen Hochschule, Schnittspahnstr. 3-5, D-6100, Darmstadt, Federal Republic of Germany.

|January 14, 2014

Summary

Duckweed (Lemna gibba) growth and respiration increase with sucrose. The plant utilizes an energy-dependent system for hexose uptake, enhanced by light.

Area of Science:

Plant Physiology
Plant Biochemistry
Photosynthesis and Respiration

Background:

Autotrophically growing duckweed (Lemna gibba L., G1) exhibits altered metabolic responses to carbohydrate availability.
Understanding sugar uptake mechanisms is crucial for comprehending plant carbon assimilation and energy metabolism.

Purpose of the Study:

To investigate the effects of sucrose and other sugars on the growth, respiration, and photosynthesis of Lemna gibba.
To elucidate the kinetics and regulation of hexose uptake in Lemna gibba under varying light and carbon conditions.

Main Methods:

Cultivation of Lemna gibba under different sucrose regimes and light conditions.
Measurement of oxygen uptake (respiration) and photosynthetic rates.
Analysis of hexose uptake kinetics using radiolabeled sugars (e.g., 3-O-methylglucose) and varying substrate concentrations.

More Related Videos

Measuring Cation Transport by Na,K- and H,K-ATPase in Xenopus Oocytes by Atomic Absorption Spectrophotometry: An Alternative to Radioisotope Assays

Measuring Cation Transport by Na,K- and H,K-ATPase in Xenopus Oocytes by Atomic Absorption Spectrophotometry: An Alternative to Radioisotope Assays

Published on: February 19, 2013

Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects

Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects

Published on: May 4, 2018

Related Experiment Videos

Last Updated: May 4, 2026

High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits

High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits

Published on: September 20, 2016

Measuring Cation Transport by Na,K- and H,K-ATPase in Xenopus Oocytes by Atomic Absorption Spectrophotometry: An Alternative to Radioisotope Assays

Measuring Cation Transport by Na,K- and H,K-ATPase in Xenopus Oocytes by Atomic Absorption Spectrophotometry: An Alternative to Radioisotope Assays

Published on: February 19, 2013

Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects

Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects

Published on: May 4, 2018

Enzyme activity assays for cell wall invertase.

Main Results:

Sucrose significantly stimulated duckweed growth and increased respiration rates.
Photosynthesis was inducible even at low light intensities (0.1 klx).
Short-term sugar application stimulated respiration proportionally to hexose uptake, indicating sucrose hydrolysis by invertase prior to uptake.
Hexose uptake was constitutive, energy-dependent, and exhibited similar saturation kinetics for glucose and 3-O-methylglucose, with light enhancing uptake by 2-3 fold.

Conclusions:

Lemna gibba possesses an energy-dependent, constitutive system for hexose uptake, crucial for its carbon metabolism.
Sucrose is likely hydrolyzed to hexoses by cell wall invertase before cellular uptake.
Light availability significantly influences the efficiency of hexose transport in duckweed.