Phytase production and decrease of phytic acid content in canola meal byAspergillus carbonarius in solid-state
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World Journal of Microbiology & Biotechnology
|January 14, 2014
Summary
Solid-state fermentation with Aspergillus carbonarius enhances canola meal protein and phytase production. Optimal conditions reduce phytic acid, improving nutritional value for animal feed applications.
Area of Science:
- Biotechnology
- Microbial Fermentation
- Agricultural Science
Background:
- Canola meal is a protein-rich byproduct of oil extraction.
- Phytic acid in plant-based feed ingredients reduces mineral bioavailability.
- Solid-state fermentation (SSF) offers a sustainable method for bioprocessing agricultural byproducts.
Purpose of the Study:
- To optimize phytase production using Aspergillus carbonarius in canola meal via SSF.
- To evaluate the impact of SSF on the nutritional composition of canola meal, specifically protein and carbohydrate content.
- To determine the optimal conditions for reducing phytic acid content in canola meal.
Main Methods:
- Solid-state fermentation (SSF) of canola meal using Aspergillus carbonarius.
- Monitoring of phytase activity, protein content, carbohydrate concentration, and phytic acid reduction over 72 hours.
- Optimization of moisture content (53-60%) and inoculum homogenization time (120 s).
- Comparison of SSF on sterile versus non-sterile canola meal.
Main Results:
- Phytase production was growth-associated, peaking at 72 hours.
- Protein content increased by 25% at 48 hours and 10% at 72 hours.
- Total carbohydrate concentration decreased by 25% by the end of fermentation.
- Optimal phytic acid reduction occurred at 53-60% moisture.
- 120 s inoculum homogenization yielded greatest biomass and lowest phytic acid.
- Sterile meal resulted in lower phytic acid content than non-sterile meal.
Conclusions:
- Solid-state fermentation with Aspergillus carbonarius effectively enhances canola meal's nutritional profile.
- Optimized SSF conditions significantly reduce phytic acid content, improving mineral bioavailability.
- This bioprocess holds potential for upgrading canola meal into a more valuable animal feed ingredient.
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