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Purification of Neurospora crassa alkaline phosphatase without DNAse activity for use in molecular biology
M Pereira1, H Pereira, G Thedei
1Departamento de Genética, FMRP-USP, Av. Bandeirantes 3900, 14049-900, Ribeirão Preto, SP, Brazil.
World Journal of Microbiology & Biotechnology
|January 14, 2014
Abstract:
Alkaline phosphatase, excreted by Neurospora crassa preg (c) and purified to apparent homogeneity by 7.5% PAGE, did not show DNAase activity and removed the terminal 5'-phosphate group from plasmid Bluescript M13(+) linearized with EcoRI. The preg (c) strain may therefore replace other sources of alkaline phosphatase for use in dephosphorylating linearized plasmidial DNA in cloning experiments.

