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Updated: May 4, 2026

Purification of Active Photosystem I-Light Harvesting Complex I from Plant Tissues
Published on: February 3, 2023
Functional mechanism of water splitting photosynthesis
1Max-Volmer-Institut für Biophysikalische und Physikalische Chemie, Technische Universität Berlin, Straße des 17. Juni 135, D-1000, Berlin 12, Germany.
Abstract:
A personal account is given on physico-chemical aspects of photosynthesis. The article starts with the way I entered the field of photosynthesis. Then, selected results from our research group are discussed. Three methods used for functional analysis in our laboratory are described: the repetitive flash spectroscopy; the electrochromic volt- and ammeter; and the membrane energization by a battery. Our subsequent studies deal with the two photoreaction centers, the primary charge separation, the plastoquinones as a transmembrane link between the two centers and the vectorial electron- and proton pathways. The results led to a picture of the elementary functional mechanism of the molecular machinery in the thylakoid membrane. The perspective then focuses on the coupling between the electric field, protons and phosphorylation. This section is followed by our observations and analysis of the mechanism of water cleavage and its coupling with the functioning of reaction center II. Finally, information is provided on structural aspects of the two reaction centers. The article ends with a retrospect.
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